Agilent Technologies has introduced a new RNA Isolation kit designed to remove up to 1,000 times more genomic DNA than competing products. The kit does not require the use of DNase enzyme, which is commonly used to remove genomic DNA but can compromise the quality of RNA.
To scientists using RNA in their experiments, genomic DNA is a significant contaminant that interferes with and affects data reliability. The Total RNA Isolation Mini Kit uses a pre-filtration column to remove genomic DNA and can reduce contamination to less than 1 femtogram per 1 nanogram of isolated RNA in selected tissues, claims Agilent.
"This minimises experimental failure in microarray and real-time PCR experiments due to impure RNA [and] saves researchers the time and cost of doing the DNase treatment," said the company.
The kit will help researchers isolate purified RNA from tissues and cells for use in procedures such as microarray-based gene expression and real-time PCR studies.
"This is one of several breakthrough bioreagent products that Agilent is developing for gene expression and proteomics solutions," said Fran DiNuzzo, vice president and general manager of Agilent's Bioreagents, Consumables and Services business. The company plans to develop new products to improve every element of the workflow, from sample preparation to microarrays, scanners and data analysis software, he added.
The company told delegates at the Chips to Hits conference in Boston, USA, that the new kit would be commercially available from1 November.