mPLC system speeds up oligo purity testing
used to assess the purity of oligonucleotides around 16 times as
quickly as conventional high performance liquid chromatography.
The company has reported a study at this week's Tides 2004 conference in Las Vegas, US, which shows that the micro parallel liquid chromatography (mPLC) product, called the Veloce, carried out purity assessment much faster than HPLC on samples in a standard 384-well microplate. This type of assessment is used in a number of molecular biology techniques such as cloning and ligation.
The data resulting from routine quality control of synthetic oligonucleotides using the Veloce system is within 4 per cent of the results obtained from HPLC - indicating that it is as reliable as the gold-standard method - and demonstrates good retention time reproducibility, efficiency and resolution, according to the firm.
The Veloce system includes instrumentation, software and 24-column microfludic Brio cartridges and uses pressure-driven flow to achieve chromatograms comparable to HPLC, with up to 24 samples being tested in parallel in a single run. It was launched earlier this year, according to Nanostream.
