Companies making drugs have been trying to reduce the use of animal products in the production environment because of the risk that these materials may carry the agents that cause transmissible spongiform encephalopathies (TSEs), such as mad cow disease and variant Creutzfeldt-Jakob diseases (vCJD) in humans.
Oxoid Cold Filterable Vegetable Peptone Broth is suitable for microbiological media fill trials (MFT) and is suitable for use as an alternative to Tryptone Soya Broth, according to the company.
In MFT testing, culture media is substituted for the usual pharmaceutical product in the production line, and goes through the usual production process, including filter sterilisation. The medium is then recovered and incubated for the recovery of contaminating bacteria, yeasts and moulds, and this provides an index of the level of microbiological contamination occurring in aseptic manufacture and can be used to identify problems in contamination control.
Formulated using a pea peptone (digested using fungal enzymes), Oxoid Cold Filterable Vegetable Peptone Broth is claimed to be a highly nutritious, general purpose medium that will support the growth of a wide range of bacteria, yeasts and moulds.
The product receives a gamma irradiated sterilising dose that is validated to be lethal for all yeasts, moulds and bacteria, including bacterial spores and mycoplasmas, said Oxoid. In addition, each component is screened and selected to ensure easy filtration, making it ideal for MFT.