Resin could replace silica for biologics polishing

Related tags Chromatography

Rohm and Haas' Advanced Biosciences division has launched a new
product in its Amberchrom range of chromatographic resins that can
replace and improve on silica in the final stages of biologics

Amberchrom HPR10 reversed phase chromatographic resin is claimed to reduce operating costs and improve process economics, by combining the advantages of both premium silica and polymeric media, including selectivity, high yield and capacity, full-range pH stability (a key advantage over silica), compatibility with clean- and sterilise-in-place (CIP/SIP) procedures and stability at pressures up to 100 bar.

The product is a 10 micron monosized polymeric resin, said to be unique in the marketplace, that has been optimised to deliver high capacity for peptides, small proteins and other biomolecules.

Previously, silica was the preferred media for final polishing steps in bioprocessing, while polymeric resins were mostly used for capture and the intermediate step. Amberchrom HPR10 resin has changed that, says Rohm and Haas, as its rigid polymeric matrix and mechanical resistance is close to that of silica, allowing it to perform at the higher pressures needed for improved selectivity and purity.

In addition, columns packed with the resin can be cleaned in place more easily than silica in the final polishing step, requiring just sodium hydroxide treatment, and this saves processing and validation time, said the company.

It also has an extended lifetime which should reduce total operating costs by decreasing plant shutdown time and overall chromatographic media costs.

"The high loading capabilities of the Amberchrom HPR10 resin enable economical, large scale operation for critical products such as therapeutic peptides,"​ said Rohm and Haas in a statement.

Amberchrom HPR10 resin is available in bulk from analytical to industrial quantities, but also as Amberchrom Profile HPR10 RP HPLC columns with a 4.6, 10, 20, 50 or 70 mm diameter.

Rohm and Haas' Amberlite and Amberchrom resins are well-established in the market for purification, solvent removal and molecule recovery, and were latterly joined by Ambersynth, a solid-phase synthesis resin which is suitable for use in processes up to commercial multi-tonne scale.

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