Upstate releases Western Blotting detection kits
detection kit, which offers the highest signal-to-noise ratios of
any existing luminal peroxidase formulations on the market,
providing improved sensitivity and low backgrounds at a more
economical price.
Upstate's latest release is based on its Visualizer Western Blot Detection Kit, incorporating its established and proven platform to provide a high glow reaction chemiluminescent substrate that is sensitive and long lasting.
"Higher reliability at a lower cost is an option our customers want," said David Dodd, president and chief executive officer of Serologicals.
"Upstate has a full pipeline of new products like Visualizer EC that will help speed research, drug discovery and bioprocessing," he added.
The kit, which is available as 20 or 50 mini-blot kits, as well as a 5 mini-blot sample kit, is aimed at biomedical researchers at drug discovery companies and academic institutions in the disciplines of oncology, immunology, proteomics, cardiology and neurology.
Western blotting has become one of the most common protein analysis techniques used in biomedical research today. It provides a non-radioactive, direct method for identifying proteins in a mixture, as well as determining the relative amount of specific proteins in different samples.
While variations on standard Western blotting techniques are numerous, its accururacy ensures it remains the most popular of the options available. In general, a complex protein mixture (such as a cell lysate, cell extract or purified protein preparation) is fractionated on a gel (e.g. SDS-PAGE, native PAGE, IEF or 2-D PAGE) by electrophoresis.
After separation, proteins are transferred to a membrane, which can be nitrocellulose or polyvinylidene fluoride (PVDF). For the best results with Visualizer EC, we recommend using PVDF. Nitrocellulose can also be used, but more cell sample is needed compared to that with PVDF.
Imaging is performed on standard X-ray film or by using an image documenting station that is fitted with a chemiluminescent- sensitive camera.
This provides the capability of making multiple exposures of a blot, securing valuable data, extending the dynamic range of detection and making quantitation easier and more accurate.
Upstate's Visualizer EC Western Blot Detection Kit uses an improved version of the chemiluminescent HRP substrate luminol that results in the most sensitive detection of an antigen.
The kit includes chemiluminescent substrates in an easy to prepare format and can be used with any HRP-Conjugated Secondary Antibody.
More details of Upstate's Visualizer EC Western Blot Detection Kit is available from the company's website.
