Biopharmaceutical production is a rapidly growing and high risk industry. According to a market research report, only three of ten drugs receive market clearance. Cell culture media plays an important role in biopharmaceutical production. Handheld Raman provides the means for non-destructive, cost effective, and efficient analysis of complex cell culture media essential for high quality process control. However, the choice of laser wavelength excitation source is paramount to achieving detailed chemical information from media samples. A study of synthetic cell culture media concluded that use of a handheld Raman analyzer equipped with a 1064nm laser wavelength revealed distinct results.