Tangential flow filtration (TFF) is widely used in the biopharmaceutical industry for downstream processing applications. Typical TFF steps concentrate product through volume reduction, and buffer exchange through diafiltration, to achieve high yields. TFF typically utilizes ultrafiltration membranes ranging from 1 – 1000 kD nominal molecular weight limit (NMWL) to retain different size molecules. Traditional TFF requires multiple passes through a system, using a pump to drive feed through a filter and sending the retentate back to a tank for another pass through the system.
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