Sigma launches FluoroProfile
approach for the fluorescent quantification of proteins offering
numerous advantages over existing kits that include reversible
covalent binding, large dynamic range, improved sensitivity, and
emission stability.
The FluoroProfile (FP0010), is designed for the determination of protein presence and uses a proprietary fluorophore epicocconone, which fluoresces intensely red on binding to proteins. Binding is reversible, rendering the proteins completely amenable to mass spectrometry, N-term sequencing and to functional assays.
The ability to bind to free amines reversibly in an aqueous environment make this the preferred method for quantification prior to mass spectrometry of precious small samples.
Tolerance of buffering and solubilising substances that interfere with other commonly used quantification methods eliminates the requirement of precipitation and risk of sample modification.
As a traditional technique, protein quantitation may be accomplished through a number of methods, the most reliable of which include the BCA (bicinchoninic acid) assay and the Bradford assay. Alternative protein quantitation methods include the Lowry assay for protein quantitation and derivitisation of N-terminal amines.
The Fluroprofile ensures there is no loss of protein sample to quantification making FluoroProfile the ideal to quantify small samples before peptide mass fingerprinting and expression analysis by mass spectrometry.
FluoroProfile is robust to substances that inhibit most protein assays and is particularly suited to quantifying proteins in samples prepared for 2-D and 1-D electrophoresis, IEF gels and anywhere accurate quantification of protein is needed.
